Superantigen stimulation

Superantigens such as Staphylococcal Enterotoxin B (SEB) activate lymphocytes very efficiently. Test your compound's immunomodulatory capacity by analyzing its effects in a superantigen assay!

The effects of immunomodulatory compounds on lymphocytes can be investigated in cultures in which lymphocytes are stimulated by superantigens. Superantigens stimulate a large number of lymphocytes, as opposed to regular antigens that only activate a handful of cells. By using suboptimal concentrations of superantigen, inhibition or stimulation of proliferation/cytokine production by your compound of interest can be determined easily.

Depending on your wishes, we can either repeatedly use the same donor samples or use numerous different donors. 

PBMCs of a donor will be stimulated with SEB in the presence or absence of your compound(s) of interest.

Advantages of superantigen stimulation

  • Antigen-independent and robust activation

  • Stimulates a large number of cells in a non-specific, polyclonal, fashion

Read-out options

We offer several different read-out options for superantigen activation, but feel free to contact us for an assay customized to your needs.

Other stimulation assays

Besides super-antigen stimulation, we also offer other stimulation assays. In addition to antibody-mediated receptor crosslinking and chemical stimulation, please find a selection of our assays below.

Contact

Need help?

Get in touch with Anja ten Brinke, PhD or Annelies Turksma, PhD
Immunomonitoring@Sanquin.nl