Cytokine analyses- ELISA / CBA

Cytokine production can be measured with different methods. With a cytokine ELISA, the concentration of cytokines in serum, plasma or culture medium is measured. The ELISA approach makes it easy to process many samples at once, in different dilutions.

This method can be used to screen for immunogenicity, reactions to antigens, T cell skewing, to identify inflammatory reactions and many more applications.

Performing ELISAs is an efficient method to test many samples in one experiment and in different dilutions. If, however, you are more interested in measuring various cytokines simultaneously in a small volume, the cytokine bead assay (CBA) may be more suitable. The CBA is read-out by flow cytometry and a small amount of sample is enough to identify many different cytokines at once.

Example

Comparison of different tolerogenic DCs.

DC- T cell MLR- cytokine production by ELISA

 

 

 

 

 

 

 

 

Different types of tolerogenic DC and mDC were incubated with allogeneic naive CD4+ T cells (ratio 1:10). Production of IL-10, IL-13 and IFN╬│ was determined by ELISA at day 6. The cultures were performed in duplicate and mean + SEM of 5 independent experiments is shown.

Reference

IL-10-generated tolerogenic dendritic cells are optimal for functional regulatory T cell induction--a comparative study of human clinical-applicable DC.
Boks MA, Kager-Groenland JR, Haasjes MS, Zwaginga JJ, van Ham SM, ten Brinke A. Clin Immunol. 2012 Mar;142(3):332-42

Contact

Need help?

Get in touch with Anja ten Brinke, PhD or Annelies Turksma, PhD
Immunomonitoring@Sanquin.nl