Lab

Kits

For Monocyte Activation Test

Sanquin’s MAT kit: A highly sensitive in vitro human cell-based assay for pyrogen detection, worldwide available through our partner Lonza

The European Pharmacopeia (EP) states that, after a product-specific validation, the Monocyte Activation Test (MAT) is suitable as a replacement for the rabbit pyrogen test.

The MAT Cell Set offers a pool of cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) with dedicated Culture Medium Supplement ensuring a high sensitivity.

Test procedure

Day 1:
Under non-pyrogenic conditions:

  • To a 96-wells plate add culture medium, endotoxin standard, product samples & MAT Cells
  • Incubate for 18-24h at 37ºC

Day 2:
Under standard lab conditions:

  • Transfer supernatant into cytokine ELISA
  • Measure cytokine levels and analyze samples

Sanquin products for MAT

For cell incubation (day 1)

MAT Cell Set for 3x 96-well plates (2 to 3 samples per plate) consisting of:

  • 3 vials of cryopreserved pooled PBMCs
  • 3 vials of dedicated MAT Culture Medium Supplement

For ELISA (day 2)

PeliKine compact IL-6 ELISA kit and PeliKine-Tool set

  • Sufficient for 3 highly sensitive ELISA plates

Why use Sanquin’s MAT kit?

  • PBMCs qualified according to European Pharmacopoeia Chapter 2.6.30, section 5
  • Limit of Detection (LOD) ≤ 0.02 EU/mL*
  • Reactive towards non-endotoxin pyrogens
  • Measurement of IL-6 as cytokine read-out
  • Proven robustness and reliability
  • Storage at -80ºC (no liquid nitrogen required)
  • Assay time 24 hours including overnight culture

Why cryopreserved pooled PBMC as cell source?

  • Comparable reactivity to fresh PBMC
  • No need for blood donors and cell isolation for each experiment
  • Pool of 4 donors taking donor variation into account
  • Available on demand
  • Storage at -80ºC (no liquid nitrogen required)
  • Proven robustness and reliability

Why IL-6 as cytokine read-out?

  • High sensitivity
  • Fully secreted into the medium
  • Stable in (frozen) supernatant

Interested? Please mail to: MAT@sanquin.nl

Hesitant to perform the assay in your own lab? Check our MAT services.

*Given as concentration per sample, i.e. prior to addition of pMAT Cells to the samples.