Kits

Sanquin’s MAT kit: A highly sensitive in vitro human cell-based assay for pyrogen detection

The European Pharmacopeia (EP) states that, after a product-specific validation, the Monocyte Activation Test (MAT) is suitable as a replacement for the rabbit pyrogen test.

The MAT Cell Set offers a pool of cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) with dedicated Culture Medium Supplement ensuring a high sensitivity.

Test procedure

Day 1:
Under non-pyrogenic conditions:

• To a 96-wells plate add culture medium, endotoxin standard, product samples & MAT Cells
• Incubate for 18-24h at 37ºC

Day 2:
Under standard lab conditions:

• Transfer supernatant into cytokine ELISA
• Measure cytokine levels and analyze samples

Sanquin products for MAT

For cell incubation (day 1)

MAT Cell Set for 3x 96-well plates (2 to 3 samples per plate) consisting of:

• 3 vials of cryopreserved pooled PBMCs
• 3 vials of dedicated MAT Culture Medium Supplement

For ELISA (day 2)

PeliKine compact IL-6 ELISA kit and PeliKine-Tool set

• Sufficient for 3 highly sensitive ELISA plates

Why use Sanquin’s MAT kit?

• PBMCs qualified according to European Pharmacopoeia Chapter 2.6.30, section 5
• Limit of Detection (LOD) ≤ 0.01 EU/mL
• Reactive towards non-endotoxin pyrogens
• Measurement of IL-6 as cytokine read-out
• Proven robustness and reliability
• Storage at -80ºC (no liquid nitrogen required)
• Assay time 24 hours including overnight culture

Why cryopreserved pooled PBMC as cell source?

• Comparable reactivity to fresh PBMC
• No need for blood donors and cell isolation for each experiment
• Pool of 4 donors taking donor variation into account
• Available on demand
• Storage at -80ºC (no liquid nitrogen required)
• Proven robustness and reliability

Why IL-6 as cytokine read-out?

• High sensitivity
• Fully secreted into the medium
• Stable in (frozen) supernatant

Interested? Please mail to: MAT@sanquin.nl

Hesitant to perform the assay in your own lab? Check our MAT services.