
Kits
For Monocyte Activation TestSanquin’s MAT kit: A highly sensitive in vitro human cell-based assay for pyrogen detection, worldwide available through our partner Lonza
The European Pharmacopeia (EP) states that, after a product-specific validation, the Monocyte Activation Test (MAT) is suitable as a replacement for the rabbit pyrogen test.
The MAT Cell Set offers a pool of cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) with dedicated Culture Medium Supplement ensuring a high sensitivity.
Test procedure 
Day 1:
Under non-pyrogenic conditions:
- To a 96-wells plate add culture medium, endotoxin standard, product samples & MAT Cells
- Incubate for 18-24h at 37ºC
Day 2:
Under standard lab conditions:
- Transfer supernatant into cytokine ELISA
- Measure cytokine levels and analyze samples
Sanquin products for MAT
For cell incubation (day 1)
MAT Cell Set for 3x 96-well plates (2 to 3 samples per plate) consisting of:
- 3 vials of cryopreserved pooled PBMCs
- 3 vials of dedicated MAT Culture Medium Supplement
For ELISA (day 2)
PeliKine compact IL-6 ELISA kit and PeliKine-Tool set
- Sufficient for 3 highly sensitive ELISA plates
Why use Sanquin’s MAT kit?
- PBMCs qualified according to European Pharmacopoeia Chapter 2.6.30, section 5
- Limit of Detection (LOD) ≤ 0.02 EU/mL*
- Reactive towards non-endotoxin pyrogens
- Measurement of IL-6 as cytokine read-out
- Proven robustness and reliability
- Storage at -80ºC (no liquid nitrogen required)
- Assay time 24 hours including overnight culture
Why cryopreserved pooled PBMC as cell source?
- Comparable reactivity to fresh PBMC
- No need for blood donors and cell isolation for each experiment
- Pool of 4 donors taking donor variation into account
- Available on demand
- Storage at -80ºC (no liquid nitrogen required)
- Proven robustness and reliability
Why IL-6 as cytokine read-out?
- High sensitivity
- Fully secreted into the medium
- Stable in (frozen) supernatant
Interested? Please mail to: MAT@sanquin.nl
Hesitant to perform the assay in your own lab? Check our MAT services.
*Given as concentration per sample, i.e. prior to addition of pMAT Cells to the samples.