If you have any questions that are not listed on this page, please contact us at MAT@sanquin.nl.
FAQFrequently asked questions related to MAT
How many samples can I run with one MAT Cell Set?
The MAT Cell Set contains 3 vials of MAT Cells with ~5 million cells per vial and 3 vials of dedicated MAT Culture Medium Supplement. A single vial contains sufficient cells to run one 96-well microtiter plate. Depending on the plate layout and European Pharmacopeia (chapter 2.6.30) method used you can run 2 or 3 samples per plate. So with one MAT Cell Set you can test 6 to 9 samples.
How should I store the MAT Cell-set?
The MAT Cell Set should be stored at ≤ -80°C in the original packaging.
Our freezer has an average temperature of -70°C, can I still store the MAT Cell Set in this freezer?
Although experimental data has shown no significant loss of cell responsiveness when stored at -70°C for 2 weeks, the MAT Cell Set should be stored at ≤ -80°C in the original packaging.
We have easy access to liquid nitrogen storage, can I store the MAT Cell Set there?
The MAT Cell Set should be stored at ≤ -80°C in the original packaging, which cannot be placed in liquid nitrogen. Although storage of MAT cells in liquid nitrogen may increase its shelf life, the MAT Cell Set may not be used beyond it expiry date, which has been determined at -80°C.
Do we need to monitor the freezer for temperature deviations?
Yes, we recommend using an alarm system to monitor the temperature. Prolonged storage > -80°C may impact the performance of the MAT Cell Set, resulting in a reduction in shelf life.
How do you ship your MAT Cell Set?
The product is shipped directly from our warehouse in Amsterdam (the Netherlands) on dry ice. A maximum of 2 MAT Cell Sets are packed per styrofoam transport box containing ~12 kg of dry ice, which is sufficient for a shipment of maximum 72 hours. If you expect that shipment may take longer than 72 hours, we recommend that you select a courier that provide dry ice refilling services.
Can I unpack the box with MAT Cell Set at room temperature in our lab?
We recommend to unpack the box with dry ice within close proximity of the ultra-low (≤ -80°C) storage freezer to allow an immediate transfer of the MAT cell Set into the freezer. Any delay causing an increase in temperature might affect the performance of the assay.
How should I best thaw the cells prior to use in MAT?
Please refer to the Instructions for Use of the MAT Cell Set for thawing instructions.
To which non-endotoxin pyrogens do the MAT cells respond?
The cells have shown responsiveness to Lipoteichoic acid (LTA) from S. aureus, Synthetic triacylated lipoprotein (Pam3CSK4), Flagellin from B. subtilis and from S. typhimurium, Heat Killed S. aureus, R848 (Resiquimod) and insoluble Peptidoglycan (PGN) from S. aureus. The cells may be responsive to other non-endotoxin pyrogens, but these have to be tested by the user. Please refer to the certificate of analysis for lot-specific information.
Can I store the culture supernatant from the MAT cells after incubation?
The culture supernatant may be frozen at -20°C or -80°C. Storage time of the frozen supernatant may be product-dependent and needs to be determined by the user.