LiteratureScientific literature related to MAT
Literature by Sanquin authors
S. Solati, L. Aarden, S. Zeerleder, D. Wouters. Innate Immunity (2015), vol 21, p667-684
. . . We conclude that the MAT, using frozen pooled PBMCs and IL-6 detection, is a highly sensitive, specific and reproducible pyrogen test that is able to detect and quantify endotoxin and non-endotoxin pyrogenic contaminations in parenteral pharmaceuticals, and replace the RPT.. . .
R. Kikkert, E.R. de Groot, L. Aarden. Journal of Immunological Methods (2008), vol 336, p45–55
. . . After stimulation (16–20 h) of cells, supernatants were harvested and stored at −20 °C until assayed. IL-1β, IL-6, IL-8, and TNF were determined by ELISA kits (PeliKine-compact, Sanquin Reagents, Amsterdam, the Netherlands). . . . we confirm that IL-6 is to be preferred over IL-1β and TNF as readout for MNC cytokine production. . . we are confident that our results will give an incentive to the area of pyrogen testing and will contribute to the ongoing discussion on alternative pyrogen testing using the monocyte activation test. . .
M.L. Helle, L. Boeije, E. de Groot, A. de Vos, L. Aarden. Journal of Immunological methods (1991), vol 138, p47-56
. . . A monoclonal antibody and an affinity purified polyclonal antibody, both raised against recombinant human IL-6, have been employed in an ELISA procedure to quantitate human IL-6. . .
Other interesting reads
Katherine Antunes de Mattos, ATLA vol 46, (2018), 23–37
. . . The quantitative analysis demonstrated the correlation between the MAT and the Limulus amoebocyte lysate (LAL) assays, ...
Johannes Reich et al. Biologicals, vol 53, (May 2018), 1-9
. . . Our work provides for the first time a model to simulate masking kinetics of endotoxin which lends a better understanding of LER. . .
C.C. da Silva, O.A.F. Presgrave, T.Hartung, A.M. Lage de Moraes, I. Fernandes Delgado, Toxicology in Vitro, vol 32 (2016) 70–75
. . . These results show that MAT may replace the use of rabbits in pyrogen tests of HS . . .
P. Bruegger, Journal of Immunological Methods, vol 405 (2014) 181–191
. . . So far the MAT is the best alternative method to the rabbit pyrogen test to detect both, endotoxin and non-endotoxin pyrogens . . .