Literature & PresentationsScientific literature & presentations related to MAT
Literature by Sanquin authors
Performance of Monocyte Activation Test Supplemented with Human Serum Compared to Fetal Bovine Serum (Altex 2020)
Marijke W. A. Molenaar-de Backer, Eelo Gitz, Miranda Dieker, Paulien Doodeman and Anja ten Brinke. ALTEX preprint published October 28, 2020 doi:10.14573/altex.2008261
… The MAT was more sensitive for endotoxin when FBS was used, however for most NEPs the MAT was more sensitive when performed in HS…
An improved monocyte activation test using cryopreserved pooled human mononuclear cells (Innate Immunity 2015)
S. Solati, L. Aarden, S. Zeerleder, D. Wouters. Innate Immunity (2015), vol 21, p667-684
. . . We conclude that the MAT, using frozen pooled PBMCs and IL-6 detection, is a highly sensitive, specific and reproducible pyrogen test that is able to detect and quantify endotoxin and non-endotoxin pyrogenic contaminations in parenteral pharmaceuticals, and replace the RPT.. . .
Cytokine induction by pyrogens: Comparison of whole blood, mononuclear cells, and TLR-transfectants ( Journal of Immunological Methods 2008)
R. Kikkert, E.R. de Groot, L. Aarden. Journal of Immunological Methods (2008), vol 336, p45–55
. . . After stimulation (16–20 h) of cells, supernatants were harvested and stored at −20 °C until assayed. IL-1β, IL-6, IL-8, and TNF were determined by ELISA kits (PeliKine-compact, Sanquin Reagents, Amsterdam, the Netherlands). . . . we confirm that IL-6 is to be preferred over IL-1β and TNF as readout for MNC cytokine production. . . we are confident that our results will give an incentive to the area of pyrogen testing and will contribute to the ongoing discussion on alternative pyrogen testing using the monocyte activation test. . . .
Sensitive ELISA for interleukin-6: Detection of IL-6 in biological fluids: synovial fluids and sera ( Journal of Immunological methods 1991)
M.L. Helle, L. Boeije, E. de Groot, A. de Vos, L. Aarden. Journal of Immunological methods (1991), vol 138, p47-56
. . . A monoclonal antibody and an affinity purified polyclonal antibody, both raised against recombinant human IL-6, have been employed in an ELISA procedure to quantitate human IL-6. . . .
Other interesting reads
Monocyte-activation test to reliably measure the pyrogenic content of a vaccine: An in vitro pyrogen test to overcome in vivo limitations (Vaccine 2019)
S. Valentini et al. , Vaccine, Volume 37, Issue 29, 27 June 2019, Pages 3754-3760
...Being aware of these limitations of the RPT and LAL when applied to Bexsero, the Monocyte Activation Test (MAT) which is sensitive to both endotoxin and non-endotoxin based pyrogens has been developed as an alternative pyrogen test....
Applicability of the Monocyte Activation Test (MAT) in the Quality Control of the 17DD Yellow Fever Vaccine ( ATLA 2018)
Katherine Antunes de Mattos et al. , ATLA vol 46, (2018), 23–37
. . . The quantitative analysis demonstrated the correlation between the MAT and the Limulus amoebocyte lysate (LAL) assays, ...
Investigation of the kinetics and mechanism of low endotoxin recovery (LER) in a matrix for biopharmaceutical drug products (Biologicals 2018)
Johannes Reich et al. Biologicals, vol 53, (May 2018), 1-9
. . . Our work provides for the first time a model to simulate masking kinetics of endotoxin which lends a better understanding of LER. . .
Applicability of the Monocyte Activation Test (MAT) for hyperimmune sera in the routine of the quality control laboratory: Comparison with the Rabbit Pyrogen Test (RPT) (Toxicology in Vitro 2016)
C.C. da Silva, O.A.F. Presgrave, T.Hartung, A.M. Lage de Moraes, I. Fernandes Delgado, Toxicology in Vitro, vol 32 (2016) 70–75
. . . These results show that MAT may replace the use of rabbits in pyrogen tests of HS . . .
Cryopreservation of human monocytes for pharmacopeial monocyte activation test (Journal of Immunological Methods 2014)
P. Bruegger, Journal of Immunological Methods, vol 405 (2014) 181–191
. . . So far the MAT is the best alternative method to the rabbit pyrogen test to detect both, endotoxin and non-endotoxin pyrogens . . .
Highly sensitive pyrogen detection on medical devices by the monocyte activation test (ournal of Materials Science: Materials in Medicine 2014)
Katerina Stang et al, Journal of Materials Science: Materials in Medicine ,volume 25, pages1065–1075(2014)
...Therefore, screening of medical devices prior clinical application using our novel assay, has the potential to significantly reduce complications associated with pyrogen-contaminated medical devices...
Presentations By Sanquin
Using the Monocyte Activation Test to determine the pyrogenicity of OMV preparations - Pharmalab 2020 - Marijke Molenaar-de Backer, PhD
Comparison of a Monocyte Activation Test based on fetal bovine serum and on human AB serum - Pharmalab 2019 - Eelo Gitz, PhD