Antigen-specific CD8+ T cell analyses

Antigen-specific CD8+ T cell analysis can be done by means of heterotetramer combinatorial coding (HTCC). HTCC allows detection and characterization of multiple antigen-specific CD8+ T cells simultaneously in a small patient sample volume. 

Heterotetramer combinatorial coding

Sanquin offers a high-throughput screening of your patient samples to detect antigen-specific T cells in vaccination studies or cell therapy.

The detection of antigen-specific CD8+ T cells recognizing an HLA binding peptide is possible by staining these cells with fluorescently labelled multimers. Thus far, the number of antigen-specific T cell populations that could be detected in a single sample sample was limited. This limits the ability to perform a comprehensive analysis of disease-associated or therapy-induced T cell responses. In particular for clinical samples this can be a considerable disadvantage.

Advantage of combinatorial coding

Combinatorial coding is a new high-tech method to circumvent the limitation of sample size. With this technique monitoring of T cell recognition of the MHC-peptide complexes can be performed quickly in small sample volumes.

41 Different MHC/peptide combinations can be analysed simultaneously in one blood sample of 4-8ml blood (instead of using more than 200ml of blood) to broadly screen for immunogenic peptides.

The combinatorial coding can be used for:

  • screening of the immunogenicity of your peptides of interest
  • detecting antigen specific T cells during clinical interventions, such as vaccination studies or cell therapy
  • combining phenotype and functional markers with antigen specificity


Need help?

Get in touch with Anja ten Brinke, PhD or Annelies Turksma, PhD