Antigen-specific CD8+ T cell analyses

Antigen-specific CD8+ T cell analysis can be done by means of heterotetramer combinatorial coding (HTCC). Combinatorial coding allows detection of antigen-specific CD8+ T cells recognizing multiple MHC-peptide complexes simultaneously in a small patient sample volume. 

Combinatorial coding

Sanquin offers a high-throughput screening of your patient samples to detect antigen-specific T cells in vaccination studies or cell therapy.

The detection of antigen-specific CD8+ T cells recognizing an HLA binding peptide is possible by staining these cells with fluorescently labelled multimers. Thus far, the number of antigen-specific T cell populations that could be detected in a single sample sample was limited. This limits the ability to perform a comprehensive analysis of disease-associated or therapy-induced T cell responses. In particular for clinical samples this can be a considerable disadvantage.

Advantage of combinatorial coding

Combinatorial coding is a new high-tech method to circumvent the limitation of sample size. With this technique monitoring of T cell recognition of the MHC-peptide complexes can be performed quickly in small sample volumes.

28 Different MHC/peptide combinations can be analysed simultaneously in one blood sample. Instead of using 200ml of blood, only 8ml of blood is needed to broadly screen for immunogenic peptides.

The combinatorial coding can be used for:

  • screening of the immunogenicity of your peptides of interest
  • detecting antigen specific T cells during clinical interventions, such as vaccination studies or cell therapy


Need help?

Get in touch with Anja ten Brinke, PhD or Annelies Turksma, PhD