Antigen-specific CD8+ T cell analyses
Antigen-specific CD8+ T cell analysis can be done by means of heterotetramer combinatorial coding (HTCC). HTCC allows detection and characterization of multiple antigen-specific CD8+ T cells simultaneously in a small patient sample volume.
Heterotetramer combinatorial coding
Sanquin offers a high-throughput screening of your patient samples to detect antigen-specific T cells in vaccination studies or cell therapy.
The detection of antigen-specific CD8+ T cells recognizing an HLA binding peptide is possible by staining these cells with fluorescently labelled multimers. Thus far, the number of antigen-specific T cell populations that could be detected in a single sample sample was limited. This limits the ability to perform a comprehensive analysis of disease-associated or therapy-induced T cell responses. In particular for clinical samples this can be a considerable disadvantage.
Advantage of combinatorial coding
Combinatorial coding is a new high-tech method to circumvent the limitation of sample size. With this technique monitoring of T cell recognition of the MHC-peptide complexes can be performed quickly in small sample volumes.
41 Different MHC/peptide combinations can be analysed simultaneously in one blood sample of 4-8ml blood (instead of using more than 200ml of blood) to broadly screen for immunogenic peptides.
The combinatorial coding can be used for:
- screening of the immunogenicity of your peptides of interest
- detecting antigen specific T cells during clinical interventions, such as vaccination studies or cell therapy
- combining phenotype and functional markers with antigen specificity