Micha (M.) Nethe PhD

Erythropoiesis in Healthy and Deregulated Hematopoiesis LinkedIn

Training

In January 2011 I received my PhD degree in the laboratory of Prof. Peter Hordijk at Sanquin Research and obtained a personal grant from the Netherlands Organisation for Scientific Research (NWO) to move to the laboratory of Prof. Ian Macara at the University of Virginia in the USA to work on mammary gland biology (Rubicon-award). In October 2013 I obtained another personal NWO grant (Veni award) to continue my work on breast cancer in the laboratory of Prof.Jos Jonkers at the NKI on the role of E-cadherin in breast cancer. During these studies I became intrigued by the marked accumulation of E-cadherin in erythroid-defined hematopoietic malignancies in bone marrow and obtained in September 2017 a JoghemVanLoghem fellowship, granting me a PI-position and a PhD-student to initiate this line of research at Sanquin Research, Landsteiner lab/AMC.

Research interests

The incidence of anaemia development, which reflects a decrease in red blood cell (RBC) production below a healthy threshold, increases upon aging and is estimated to affect a third of the global population. Epidemiologic studies report that anaemia in elderly accelerates cognitive decline, contributes to cardiovascular diseases and in addition reduces the quality of life. Anaemia is also a hallmark of hematopoietic malignancies like myeloid dysplasia syndrome (MDS). MDS reflects a heterogeneous group of hematopoietic neoplasms in the bone marrow, diagnosed in ~500 patients a year in the Netherlands, that harbors the potential to develop into acute myeloid leukaemia (AML). Nevertheless MDS patients primarily decease due impaired treatment responses to the developed anaemia. Therefore improved insights into anaemia development are needed to develop novel treatment options and improve clinical outcome. My research group aims to identify treatable causes of anaemia by improving our insights into the regulation of red blood cell formation. Since the start of my group in January 2018, we set-up two research lines to improve our insights into RBC deregulation during anaemia formation “Identification of the role of E-cadherin during haematopoiesis in human bone marrow” and “Genome-wide screening using Crispr-Cas9 to identify novel critical regulators of erythropoiesis”.   

                                                                      

Technology

To improve our understanding into erythropoiesis in healthy and malignant bone marrow my research group uses optimized in vitro culture systems to interrogate erythropoiesis in healthy and malignant human-derived bone marrow tissue which we combine with state-of-the-art in vivo models. In addition we are using innovative imaging techniques such as 8-color-immunofluorescence and intravital imaging as well as advanced biomolecular techniques such as CrisprCas9 technology.

Resume

 

 

2018 – present

 

Group Leader at Sanquin Research

 

2013 – 2018

 

Senior post-doc, Department of Molecular Pathology, Netherlands Cancer Institute (Chief; Jos Jonkers)

 

2011 – 2013

 

Post-doc, Department of Cell and Development Biology, University of

Charlottesville, Charlottesville, USA (Chief; Ian Macara)

 

2005 – 2010

 

PhD, Department of Molecular Cell Biology, Sanquin (Chief; Peter Hordijk)

Other publications

Kas SM, de Ruiter JR, Schipper K, Annunziato S, Schut E, Klarenbeek S, Drenth AP, van der Burg E, Klijn C, Ten Hoeve JJ, Adams DJ, Koudijs MJ, Wesseling J, Nethe M, Wessels LFA#, Jonkers J#. Insertional mutagenesis identifies drivers of a novel oncogenic pathway in invasive lobular breast carcinoma.
Nat Genet. 2017 Aug;49(8):1219-1230                                                              
                          
Boelens M1, Nethe M1, Klarenbeek S1, de Ruiter JR, Schut E, Bonzanni N, Zeeman AL,  Wientjens E, van der Burg E, Wessels L, Amerongen R, Jonkers J. PTEN loss rescues apoptosis in E-cadherin deficient mammary epithelial cells, resulting in development of classical invasive lobular carcinoma in mice.
Cell Reports 2016 Aug 23;16(8):2087-2101                                             

Annunziato S1, Kas SM1, Nethe M2, Yücel H, Del Bravo J, Pritchard C, Bin Ali R, van Gerwen B, Siteur B, Drenth AP, Schut E, van de Ven M, Boelens MC, Klarenbeek S, Huijbers IJ, van Miltenburg MH, Jonkers J. Modeling invasive lobular breast carcinoma by CRISPR/Cas9-mediated somatic genome editing of the mammary gland.
Genes Dev. 2016 Jun 15;30(12):1470-80.                                                          

Harthoorn LF, Sañé A, Nethe M, Van Heerikhuize JJ. Multi-transcriptional profiling of melanin-concentrating hormone and orexin-containing neurons.
Cell Mol Neurobiol. 2005. Dec;25(8):1209-23                                 

Nethe M, Berkhout B, van der Kuyl AC. Retroviral superinfection resistance.
Retrovirology. 2005 Aug 18;2:52