Julie (J) van Coillie PhD

Training

Biochemistry and Biotechnology

Research interests

Structural and functional characteristics of antibodies against membrane-embedded epitopes, more specifically antibody Fc glycosylation

Technology

• Mass spectrometry
• gene editing
• protein expression and purification
• mammalian cell work
• general molecular techniques

Resume

2022- current	Postdoctoral fellow at the Immunoglobulin Reseach group, Sanquin Research and Landsteiner Laboratory, The Netherlands
2017-2021	PhD in Glycobiology, Copenhagen Center for Glycomics, University of Copenhagen, Denmark PhD Title: The role of N-glycosylation in Fc gamma Receptor IIIa interaction with IgG – a systematic study employing genetic glycoengineering
2015-2017	Master of Science in Biochemistry and Biotechnology, University of Ghent, Belgium -    Major in Biochemistry and Structural Biology -    Minor in Biomedical Biotechnology
2012-2015	Bachelor of Science in Biochemistry and Biotechnology, University of Ghent, Belgium

 

 

 

 

Key publications

Narimatsu Y, Joshi HJ, Nason R, Van Coillie J, Karlsson R, Sun L, Ye Z, Chen YH, Schjoldager KT, Steentoft C, Furukawa S, Bensing BA, Sullam PM, Thompson AJ, Paulson JC, Büll C, Adema GJ, Mandel U, Hansen L, Bennett EP, Varki A, Vakhrushev SY, Yang Z, Clausen H. An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells. Mol Cell. 2019 Jul 25;75(2):394-407.e5. doi: 10.1016/j.molcel.2019.05.017. Epub 2019 Jun 18.  Abstract

Tajadura-Ortega V, Gambardella G, Skinner A, Halim A, Van Coillie J, Schjoldager KTG, Beatson R, Graham R, Achkova D, Taylor-Papadimitriou J, Ciccarelli FD, Burchell JM. O-linked mucin-type glycosylation regulates the transcriptional programme downstream of EGFR. Glycobiology. 2021 Apr 1;31(3):200-210. Abstract

Tian W, Ye Z, Wang S, Schulz MA, Van Coillie J, Sun L, Chen YH, Narimatsu Y, Hansen L, Kristensen C, Mandel U, Bennett EP, Jabbarzadeh-Tabrizi S, Schiffmann R, Shen JS, Vakhrushev SY, Clausen H, Yang Z. The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells. Nat Commun. 2019 Apr 30;10(1):1785. doi: 10.1038/s41467-019-09809-3. Abstract

Steentoft C, Fuhrmann M, Battisti F, Van Coillie J, Madsen TD, Campos D, Halim A, Vakhrushev SY, Joshi HJ, Schreiber H, Mandel U, Narimatsu Y. A strategy for generating cancer-specific monoclonal antibodies to aberrant O-glycoproteins: identification of a novel dysadherin-Tn antibody. Glycobiology. 2019 Apr 1;29(4):307-319. Abstract

Schulz MA, Tian W, Mao Y, Van Coillie J, Sun L, Larsen JS, Chen YH, Kristensen C, Vakhrushev SY, Clausen H, Yang Z. Glycoengineering design options for IgG1 in CHO cells using precise gene editing. Glycobiology. 2018 Jul 1;28(7):542-549. Abstract

Büll C, Nason R, Sun L, Van Coillie J, Madriz Sørensen D, Moons SJ, Yang Z, Arbitman S, Fernandes SM, Furukawa S, McBride R, Nycholat CM, Adema GJ, Paulson JC, Schnaar RL, Boltje TJ, Clausen H, Narimatsu Y. Probing the binding specificities of human Siglecs by cell-based glycan arrays. Proc Natl Acad Sci U S A. 2021 Apr 27;118(17):e2026102118.  Abstract