Monocyte Activation Test (MAT)
Sanquin Reagents has developed a reliable and reproducible Monocyte Activation Test (MAT) that can replace rabbit testing for measuring endotoxin and non-endotoxin pyrogens.
Sanquin Reagents closely co-operates with the divisions Research, Diagnostic Services, Plasma Products and Blood Bank. This combination of expertise has resulted in a unique setting for the development, production and support of MAT, which will be made available for your needs both in the context of an assay kit and services. Sanquin welcomes the opportunity to discuss with you how to apply our MAT assay, knowledge and expertise for your company’s benefit.
- Cells qualified according to European Pharmacopoeia Chapter 2.6.30
- High sensitivity and specificity
- Validated with IL-6 for LPS and several non-endotoxin pyrogens
- Proven robustness and reliability
- MAT Cell Set that consists of three vials of frozen PBMCs from four donors and dedicated MAT Culture Medium Supplement for three plates
- PeliKine compact IL-6 ELISA and toolset sufficient for three high-sensitive ELISA plates
- MAT test service Our division Diagnostic Services will be pleased to assist you from product pre-validation to routine batch release testing
- MAT Research Expertise available to solve your complex problems which may arise due to endotoxin inhibition, complex matrices, etc
MAT assay in your lab?
- The MAT assay requires expert skills and facilities to work non-pyrogenic with cell cultures and with ELISA. In case you do not have these capabilities in-house we advise our diagnostic services.
MAT relevant for
- Service testing and Diagnostic laboratories
- Pharmaceutical Companies
- Biotech Companies
- An improved monocyte activation test using cryopreserved pooled human mononuclear cells, Solati S, Aarden L, Zeerleder S, Wouters D, Innate Immunity 2015;21:667-684.
- Cytokine induction by pyrogens: comparison of whole blood, mononuclear cells, and TLR-transfectants, Kikkert R, de Groot ER, Aarden L, J Immunol Methods 2008;336:45–55.
- Sensitive ELISA for interleukin-6. Detection of IL-6 in biological fluids: synovial fluids and sera, Helle ML, Boeije L, de Groot E, de Vos A, Aarden L, J Immunol Methods 1991;138:47-56.
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