Sietse (S. Q.) Nagelkerke MD
Training
Medicine
Research interests
The role of Fc-gamma receptors in the clearance of platelets in platelet transfusion refractoriness. When patients receive a transfusion with platelets, the platelet count does not always increase adequately, a condition that is called platelet transfusion refractoriness. This problem is often caused by allo-antibodies against the transfused platelets. But even in the presence of allo-antibodies, yields of platelet transfusion differ greatly between patients. We hypothesize that these differences could be caused by polymorphisms in Fc-gamma receptors, leukocyte receptors for immunoglobin G. We are genotyping a group of patients with platelet transfusion refractoriness to be able to compare their Fc-gamma receptor profile to a control group. Also, we are setting up in vitro functional assays of phagocytosis with monocyte-derived and spleen macrophages, by which we can have a closer look at the contribution of different Fc-gamma receptors to phagocytosis of IgG-opsonised particles.
Technology
- Flow Cytometry
- Expression patterns
- Phagocytosis assays
- Cell culturing
- DNA analysis: MLPA
- qRT-PCR
Resume
| 2010-present | PhD student at Dept Blood Cell research, Sanquin, Amsterdam, The Netherlands |
| 2009-2010 | Registrar paediatrics at the Academic Medical Center, Amsterdam |
| 2005-2006 | Research project at Shaw Warren laboratory, Department of Infectious Disease, Massachusetts General Hospital, Boston MA, USA |
| 2001-2008 | Medicine, Academisch Medisch Centrum |
