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Ilse (I.) Timmerman PhD

Training

Oncology & Vascular Cell Biology 

Thesis

2013, University of Amsterdam, The Netherlands
'Dynamic control of VE-cadherin-based junctions'
 

Research interests

Cell-cell adhesion within the context of vascular tissue has been my major scientific interest in the past years. I identified several signaling cascades that target the central component of endothelial cell-cell adhesions, VE-cadherin, and thereby control vascular integrity, angiogenesis and leukocyte extravasation. To shift my focus to more translational research I decided to broaden my scientific scope to cell-cell adhesion in relation to cancer, particularly on interactions within the bone marrow microenvironment, comprising endothelial cells and mesenchymal stromal cells (MSC). I’m currently studying the cross-talk between MSC and tumor cells in bone marrow of patients with neuroblastoma. 

Technology

  • In vitro adhesion, migration and angiogenesis assays
  • In vivo vascular permeability assays
  • Colony-Forming Unit assays
  • Flow cytometry, immunoprecipitation, western blotting, antibody production and purification
  • Confocal microscopy and live cell imaging

Resume

2015-present Postdoctoral Researcher,  Dept of Hematopoiesis, Lab for Stem Cell Research, Sanquin. Topic: Bone marrow microenvironment in neuroblastoma patients
2013-2015 Postdoctoral researcher, Dept of Vascular Cell Biology, Vestweber-lab, Max Planck Institute for Molecular Biomedicine Muenster, Germany. Research topic: Regulation of vascular integrity in vivo
2008-2013 PhD student, Dept of Molecular Cell Biology, Sanquin. Research topic: Control of vascular permeability, leukocyte extravasation and angiogenesis by Vascular Endothelial (VE)-Cadherin.
2006-2008 Master of Science in Oncology, VU University, Amsterdam, The Netherlands

Other publications

Overmeer RM, Henken FE, Bierkens M, Wilting SM, Timmerman I, Meijer CJ, Snijders PJ, Steenbergen RD. Repression of MAL tumour suppressor activity by promoter methylation during cervical carcinogenesis. J Pathol 2009; 219(3):327-36.