Ilse (I.) Timmerman PhD
Oncology & Vascular Cell Biology
2013, University of Amsterdam, The Netherlands
'Dynamic control of VE-cadherin-based junctions'
Cell-cell adhesion within the context of vascular tissue has been my major scientific interest in the past years. I identified several signaling cascades that target the central component of endothelial cell-cell adhesions, VE-cadherin, and thereby control vascular integrity, angiogenesis and leukocyte extravasation. To shift my focus to more translational research I decided to broaden my scientific scope to cell-cell adhesion in relation to cancer, particularly on interactions within the bone marrow microenvironment, comprising endothelial cells and mesenchymal stromal cells (MSC). I’m currently studying the cross-talk between MSC and tumor cells in bone marrow of patients with neuroblastoma.
- In vitro adhesion, migration and angiogenesis assays
- In vivo vascular permeability assays
- Colony-Forming Unit assays
- Flow cytometry, immunoprecipitation, western blotting, antibody production and purification
- Confocal microscopy and live cell imaging
|2015-present||Postdoctoral Researcher, Dept of Hematopoiesis, Lab for Stem Cell Research, Sanquin. Topic: Bone marrow microenvironment in neuroblastoma patients|
|2013-2015||Postdoctoral researcher, Dept of Vascular Cell Biology, Vestweber-lab, Max Planck Institute for Molecular Biomedicine Muenster, Germany. Research topic: Regulation of vascular integrity in vivo|
|2008-2013||PhD student, Dept of Molecular Cell Biology, Sanquin. Research topic: Control of vascular permeability, leukocyte extravasation and angiogenesis by Vascular Endothelial (VE)-Cadherin.|
|2006-2008||Master of Science in Oncology, VU University, Amsterdam, The Netherlands|
Overmeer RM, Henken FE, Bierkens M, Wilting SM, Timmerman I, Meijer CJ, Snijders PJ, Steenbergen RD. Repression of MAL tumour suppressor activity by promoter methylation during cervical carcinogenesis. J Pathol 2009; 219(3):327-36.