Sanquin EBL outgrowth

1. Akker The majority of the in vitro erythroid expansion potential resides in CD34(−) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples. Haematologica, 2010 Sep. 95 (9), 1594–1598. [Pubmed]

2. Heideveld CD14+ cells from peripheral blood positively regulate hematopoietic stem and progenitor cell survival resulting in increased erythroid yield. Haematologica, 2015 Nov. 100 (11), 1396–1406. [Pubmed]

3. Heshusius Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells. Blood Adv. 2019 Nov 12;3(21):3337-3350. [Pubmed]

Sanquin iPSC differentiation and reviews

4. Hansen Human-induced pluripotent stem cell-derived blood products: state of the art and future directions. FEBS Lett. 2019 Sep 14. [Pubmed]

5. Hansen, Varga, Aarts Efficient production of erythroid, megakaryocytic and myeloid cells, using single cell-derived iPSC colony differentiation. Stem Cell Res. 2018 May;29:232-244. [Pubmed]

6. Varga, Hansen Erythropoiesis and Megakaryopoiesis in a Dish. IntechOpen, November 5th 2018, Book chapter [Reference Link]

Sanquin iPSC lines

7. Hansen Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts. Stem Cell Res. 2017 Jan;18:26-28. [Pubmed]

8. Hansen, Varga Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts. Stem Cell Res. 2017 Dec;25:42-45. [Pubmed]

9. Hansen, Varga Generation and characterization of a human iPSC line SANi005-A containing the gray platelet associated heterozygous mutation p.Q287* in GFI1B. Stem Cell Res. 2017 Dec;25:34-37. [Pubmed]

10. Varga, Hansen Generation of human erythroblast-derived iPSC line using episomal reprogramming system. Stem Cell Res. 2017 Dec;25:30-33. [Pubmed]

Selected publication using iPSC lines derived at Sanquin

11. Mandoli The Hematopoietic Transcription Factors RUNX1 and ERG Prevent AML1-ETO Oncogene Overexpression and Onset of the Apoptosis Program in t(8;21) AMLs. Cell Rep. 2016 Nov 15;17(8):2087-2100. [Pubmed]

12. Oorschot Molecular mechanisms of bleeding disorder associated GFI1BQ287* mutation and its affected pathways in megakaryocytes and platelets. Haematologica. 2019 Jul;104(7):1460-1472. [Pubmed]

13. Theil Trichothiodystrophy causative TFIIEβ mutation affects transcription in highly differentiated tissue. Hum Mol Genet. 2017 Dec 1;26(23):4689-4698. [Pubmed]

14. Yi CBFβ-MYH11 interferes with megakaryocyte differentiation via modulating a gene program that includes GATA2 and KLF1. Blood Cancer J. 2019 Mar 8;9(3):33. [Pubmed]


15. Takahashi and Yamanaka. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25;126(4):663-76. [Pubmed]

16. Takahashi Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30;131(5):861-72. [Pubmed]

17. Nishimura Development of defective and persistent Sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming. J Biol Chem. 2011 Feb 11;286(6):4760-71. [Pubmed]