Klaas (K. P. J. M.) van Gisbergen PhD
DC-SIGN mediates cellular communication of dendritic cells
Promotor: Prof Y van Kooyk PhD
Copromotor: TBH Geijtenbeek PhD
My research interests are aimed at understanding costimulation and transcriptional control of T cell differentiation. We are studying the costimulatory molecules CD70 and CD27 in CD8 T cell responses using CD27 KO and CD70 Tg mice. Currently, we are investigating how costimulation through CD70 and CD27 maintains clonal diversity in CD8 T cell responses. We are also investigating the role of an undescribed transcription factor that we picked up in a screen of human CD8 T cells to be upregulated in the effector compartment. We named this transcription factor Hobit and found that expression was limited to cytotoxic cells (NK cells, effector CD8 T cells and cytotoxic CD4 T cells). In mice expression was much more restricted and only found in NKT cells. We are pursuing the role of Hobit in human studies and in mouse studies using newly developed Hobit KO mice.
- Infection models in the mouse (Influenza, mCMV and LCMV)
- Flow cytometry
- Cell sorting
|2016-present||Group leader at Sanquin Research|
|2011-present||Postdoc at Dept of Hematopoiesis, Sanquin Research|
|2006-2010||Postdoc at Dept of Experimental Immunology, AMC|
|2001-2005||PhD student at Dept of Molecular Cell Biology, VU medical center|
|1996-2001||Student at Radboud University Nijmegen|
Wensveen FM, van Gisbergen KP, Derks IA, Gerlach C , Schumacher TN, van Lier RA and Eldering E. Apoptosis threshold set by Noxa and Mcl-1 after T cell activation regulates competitive selection of high-affinity clones. Immunity 2010; 32:754-65.
De Bruin AM, Buitenhuis M, van der Sluijs KF, van Gisbergen KP, Boon L, Nolte MA. Eosinophil differentiation in the bone marrow is inhibited by T cell-derived IFN-gamma. Blood 2010; 116:2559-69.
Beishuizen CR, Kragten NA, Boon L, Nolte MA, van Lier RA, van Gisbergen KP. Chronic CD70-driven costimulation impairs IgG responses by instructing T cells to inhibit germinal center B cell formation through FasL-Fas interactions. J Immunol 2009; 183: 6442-51.
Van Olffen RW, Koning N, van Gisbergen KP, Wensveen FM, Hoek RM, Boon L, Hamann J, van Lier RA, Nolte MA. GITR triggering induces expansion of both effector and regulatory CD4+ T cells in vivo. J Immunol 2009; 182:7490-500.
Nolte MA, van Olffen RW, van Gisbergen KP, van Lier RA. Timing and tuning of CD27-CD70 interactions: the impact of signal strength in setting the balance between adaptive responses and immunopathology. Immunol Rev 2009; 229:216-31.
Van Gisbergen KP, van Olffen RW, van Beek J, van der Sluijs KF, Arens R, Nolte MA, van Lier RA. Protective CD8 T cell memory is impaired during chronic CD70-driven costimulation. J Immunol 2009;182:5352-62.
Van Gisbergen KP, Ludwig IS, Geijtenbeek TB, van Kooyk Y. Interactions of DC-SIGN with Mac-1 and CEACAM1 regulate contact between dendritic cells and neutrophils. FEBS Lett 2005; 579:6159-68.
Van Gisbergen KP, Geijtenbeek TB, van Kooyk Y. Close encounters of neutrophils and DCs. Trends Immunol 2005; 26:626-31.
Van Gisbergen KP, Aarnoudse CA, Meijer GA, Geijtenbeek TB, van Kooyk Y. Dendritic cells recognize tumor-specific glycosylation of carcinoembryonic antigen on colorectal cancer cells through dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin. Cancer Res 2005; 65:5935-44.
Van Gisbergen KP, Sanchez-Hernandez M, Geijtenbeek TB, van Kooyk Y. Neutrophils mediate immune modulation of dendritic cells through glycosylation-dependent interactions between Mac-1 and DC-SIGN. J Exp Med 2005; 201:1281-92.
Van Gisbergen KP, Paessens LC, Geijtenbeek TB, van Kooyk Y. Molecular mechanisms that set the stage for DC-T cell engagement. Immunol Lett 2005; 97:199-208.
Koppel EA, van Gisbergen KP, Geijtenbeek TB, van Kooyk Y. Distinct functions of DC-SIGN and its homologues L-SIGN (DC-SIGNR) and mSIGNR1 in pathogen recognition and immune regulation. Cell Microbiol 2005; 7: 157-65.