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Maaike (M.) Schillemans

Trafficking and secretion in the vasculature LinkedIn

Training

Biomolecular Sciences, specializations Molecular Cell Biology and Biochemistry

Research interests

I am a PhD student working on a project entitled “Molecular Mechanisms of Weibel-Palade Body Exocytosis”. As the title suggests I aim to unravel the mechanisms behind the release of these endothelial secretory vesicles, with a current focus on the role of various SNARE complex and related proteins. I study the roles of these proteins in endothelial cells using various knockdown techniques such as short-hairpin RNAs and CRISPR/Cas9, and in blood outgrowth endothelial cells from patients with a mutation in my (SNARE) proteins of interest.

Technology

CRISPR/Cas9, (confocal) microscopy, mass spectrometry (proteomics/interactomics), secretion assays, FACS.

Resume

2014-present PhD student at Dept of Plasma Proteins, Sanquin Research, Amsterdam
2014 Internship at Sanquin Research Amsterdam, Dept of Plasma Proteins
Title: Impact of Selected miRNAs on the Proteome of Endothelial Cells
2013 Internship at VU University Amsterdam, Dept Molecular Cell Biology (Section Molecular Microbiology)
Title: Autotransporter Mediated Display and Secretion of Heterologous Proteins
2012-2014 Master Biomolecular Sciences, VU University, Amsterdam
2009-2012 Bachelor Human Movement Sciences, VU University, Amsterdam