Cellbind, a micro column immunofixation system

The Cellbind Screen assay is a micro column test system in which sensitized red cells from a suspension are caught by a gel matrix containing anti-IgG, anti-IgM, and anti-C3d in an enhancing high-density medium.

Benefits of Cellbind

The strongest advantages of Cellbind are the flexibility and the easy readable outcome.

1. Flexibility
Cellbind Screen is intended for use in the detection or identification of red cell antibodies as well as for blood grouping, cross-matching and the modified direct antiglobulin test (DAT, for the detection of in vivo coating of red blood cells with antibodies and complement components).

2. Easy to read
In positive reactions, red blood cells will be caught in the top layer of the gel matrix. In negative reactions, only a discrete button of red cells at the bottom of of the micro column will be seen. Reading of Cellbind cards, by eye or with a reader, is very easy and unambiguous.

3. Easy for automation
Cellbind Screen is suitable for use in manual as well as (semi-)automated systems.
The test is based on the immunofixation of sensitized red blood cells in a micro column containing a gel matrix. The cell suspension is added to the incubation compartment of the micro column, together with the plasma, serum or blood grouping reagent to be tested.

Procedure

During the incubation phase antigen-positive red blood cells will bind the corresponding anti-red blood cell antibodies present in the plasma, serum or reagent.

Next, the cards are subjected to three phases of centrifugation:

  • In the first phase optimally sensitized red blood cells are caught by ligands: anti-IgG, anti-IgM and anti-C3d in an enhancing High Density Medium. This results in a 3-dimensional network of red blood cells, attached to each other by these ligands.
  • In the second phase this network is compressed into aggregates during landing on the surface of the gel.
  • In the third phase non-sensitized red blood cells will move towards the bottom of the micro column.

For an overview of all reagents that have been validated for Cellbind use please click here.

Read more about all our products in our Serology & Immunology product catalogus 2019.

Contact us for more information or a powerpoint presentation of Cellbind at reagents@sanquin.nl.